Rapid Complement Fixation Technique for Estimating Complement-Fixing Antigen Elution Profiles of Viruses from Gel Filtration Columns

Rapid complement fixation technique for estimating complement-fixing antigen elution profiles of viruses from gel filtration columns.

The complement fixation elution profiles of dengue-2 virus-infected suckling mouse brain supernatant fluids from Sephadex G-200 columns were compared by the antigen end-point titration procedure and by a complement dilution technique. The latter technique was found to be a quick method for approximating the complement-fixing antigen in eluates.

pH Resistance of Foot-and-Mouth Disease Virus and its Complement-Fixing Antigen

Complement-Fixation Technique: II. THE TITRATION OF WASSERMANN ANTIGEN.

After material has been produced for use as an antigen in a complement fixation test it is necessary to find out if the finished product has any complement-fixing ability in the presence of a serum containing a suitable antibody. Moreover, the proposed antigen must not have any undue anticomplementary property, and the " gap" between its anticomplementary titre and its complement fixing titre m...

Complement Fixation with the Neurotropic Viruses

Antigens capable of fixing complement specifically with the appropriate antibodies have been prepared from brain tissue of hamsters and mice infected with the viruses of St. Louis, Japanese, Western, and Eastern encephalitis, and with the West Nile virus. The antigens were freed of the material which reacts with normal serum by means of centrifugation at relatively high speed. In addition, the ...

STUDIES ON INFLUENZA VIRUS THIE COmPLEMENT-FIXING ANTIGEN OF INFLUENZA A AND SWINE INFLUENZA VIRUSES

Complement fixation studies on influenza A virus (1) infections have been conducted chiefly with human sera and antigens derived from influenza mouse lungs. The use of this test to assay ferret immune sera, however, seems to have been hampered by the occurrence of non-specific or heterophile reactions (2) when such sera were tested against mouse lung suspensions. Since complement fixation offer...